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The pBluescript II vectors have a BssH II site outside each RNA promoter. .... in pBluescript II vectors is advantageous because the mutagenized insert is ...

http://www.stratagene.com/manuals/212205.pdf

EcoR I cohesive ends of the predigested pBluescript II RI vector. ... (Number of base pairs of insert) ( 0.02 g of pBluescript vector) ...

http://www.stratagene.com/manuals/212250.pdf

pBluescript vectors As pUC. Stratagene. Single-stranded replication origin. T7 and SP6 promoters flanking MCSa. pACYC vectors ...

http://www.springerlink.com/index/pp24653264388g30.pdf

cloning vector pBluescript KS (Seo et al. 2000,. 2002). The lsrA gene was highly expressed in the. E. coli clone harboring pRL1CP in a constitutive manner. ...

http://www.springerlink.com/index/w627786n11783317.pdf

The pBluescript II SK(+) vector map is adapted from http://www. stratagene.com/vectors. The nucleotide sequences relevant to this study are depicted with ...

http://www.biosci.ohio-state.edu/~vgopalan/file/4A.PDF

by RS Yadava - 2000 - Related articles

http://www.horizonpress.com/mbt/v/v1/02.pdf

by O BRAISSANT - Cited by 204

http://www.roche-applied-science.com/PROD_INF/BIOCHEMI/no1_98/p10.pdf

scribed pBluescript plasmid vectors containing either the AD169- or Towne- derived CMV UL54 DNA polymerase genes with engineered PmeI restriction ...

http://aac.asm.org/cgi/reprint/51/1/89.pdf

pBluescript plasmid vectors containing the mutation of interest with PmeI-digested T2211. 2. (AD169-SEAP) or T1472 Towne recombinant virus, respectively. ...

http://aac.asm.org/cgi/reprint/AAC.00633-06v1.pdf

ing enzymes and pBluescript SK+ vectors were purchased from Takara Shuzo (Kyoto), Nippon Gene (Toyama), and. Toyobo (Osaka). Immunostain HRP and FITC- ...

http://jb.oxfordjournals.org/cgi/reprint/111/1/20.pdf

linker of pBluescript cloning vector hybridizes with a characteristic pattern to embryonic rat ... pBluescript vectors, we show that the region between ...

http://www.jhc.org/cgi/reprint/48/7/955.pdf

enzyme and subcloned into pBluescript SK vector at EcoRV site. The resulting plasmid template was linearized with EcoRI and the anti- ...

http://www.jbc.org/content/274/18/12827.full.pdf

Construction of Plasmids, Dansfer Vectors, and Recombinant. Baculouiruses-The CPP32 cDNA was excised from the pBluescript I1. SK- vector as a 1-kilobase ...

http://www.jbc.org/content/269/49/30761.full.pdf

pBluescript vectors, was grown in Luria Bertani (LB) medium or LB solidified with agar 1.5% w/v [20]. Plasmid pBluescript I1 SKS was from Stratagene. ...

http://jmm.sgmjournals.org/cgi/reprint/45/1/57.pdf

vector is typically a pBluescript KS+ vector with two lox cassette acceptor sites flanking positive and negative selection cassettes for recombineering, ES ...

http://www.betacell.org/documents/administered/resources/data/research/ESPRESSO//Protocols/Recombineering_Protocol_for_Constructing_a_Gene_Targeting_Vector_for_RMCE.pdf

into yeast cloning vectors, we have constructed a set of YIP and YCp vectors based on the backbone of. pBLUESCRIPT. These new vectors are small (56 ...

http://www.genetics.org/cgi/reprint/122/1/19.pdf

62 kDa (81 kDa) subunit. 6223 bp. 3472 bp. 1882 bp. 925 bp linearized plasmid vector: pBluescript II. 2 kb insert. 1 kb insert ...

http://scholar.lib.vt.edu/theses/available/etd-1513132149731401/unrestricted/f17.pdf

MCS from the pBluescript SK- vector flanked by 2 I-SceI sites was inserted in the XhoI (Klenow blunted) site between the Tol2 ...

http://www.pnas.org/content/suppl/2009/07/23/0903060106.DCSupplemental/0903060106SI.pdf

and subcloned into pUC19 and pBluescript vectors and sequenced (11). The sequence obtained included 1392 nucle- otides upstream and 2498 nucleotides ...

http://www.pnas.org/content/88/5/1686.full.pdf

pBluescript KS() vector as a template for the RNA probe. For in vitro transcription, the vectors containing the 289-bp rat FLRG cDNA and 393- ...

http://www.biolreprod.org/content/68/1/199.full.pdf

ated in vectors with M13 or pBluescript primer sites. This excluded some of the early cDNA clones (Ber- natzky and Tanksley 1986) because they were gen- ...

http://genome.cshlp.org/content/8/8/842.full.pdf

again… • Retrieval vector. We use a pBluescript-based vector (PL253 or similar) or a pBR322-based vector that already contains a TK cassette for negative ...

http://recombineering.ncifcrf.gov/protocol/Protocol4_CKO_design.pdf

Additional features of the pBluescript II vectors are common to the original pBluescript phagemids (ref.2) and Include: (1) Single strand DNA rescue of ...

http://nar.oxfordjournals.org/cgi/reprint/17/22/9494.pdf

site in the polylinker of the pBluescript SK+ vector. These plasmids were called pHEN and pMEN. pLNX. This plasmid was constructed by removing the BstBI to ...

http://nar.oxfordjournals.org/cgi/reprint/21/15/3451.pdf

adipocyte RNA and inserted in the NdeI and SalI sites of hHSL(1.3)/pBluescript (SK), obtaining a full length human HSL cDNA. One vector contained the normal ...

http://www.jlr.org/cgi/reprint/M500081-JLR200v1.pdf

transcribed from pBlueScript vectors using T7 or. T3 RNA polymerase to a specific activity of 2109. c.p.m./µg. The single-stranded RNA probes were ...

http://jme.endocrinology-journals.org/cgi/reprint/21/3/277.pdf

following primers using pBluescript vectors containing the human H1 ... ligated into the pBluescript vectors. The entire coding region, including ...

http://jpet.aspetjournals.org/content/309/2/786.full.pdf

29 Jan 2004 ... H2-receptor gene were subcloned into a pBluescript SK(+) vector (La ... with the following primers using pBluescript vectors containing the ...

http://jpet.aspetjournals.org/cgi/reprint/jpet.103.063065v1.pdf

script vectors. DNA sequencing. Inserts from recombinant pBluescript vectors ... products were subcloned in pBluescript vectors and several ...

http://bloodjournal.hematologylibrary.org/cgi/reprint/86/3/1196.pdf

by MY Lin - 1996 - Cited by 20

http://ipac.kacst.edu.sa/eDoc/cloning/162660_1.pdf

subcloned into the pBluescript SK vector (Stratagene). A similar strategy as described previously (8) was used to generate a targeting vector. Briefly, a ...

http://cancerres.aacrjournals.org/cgi/reprint/62/18/5134.pdf

EcoRl site of the plasmid vector pBluescript KS*. The 0.6-kb promoter fragment was PCR amplified from human genomic DNA by using oligos ...

http://cancerres.aacrjournals.org/cgi/reprint/55/21/4999.pdf

subunits12 in the pBluescript SK vector, cDNAs of the rat GluR6 and KA2 kainate receptor subunits13 in. pGEM-HE and pBluescript SK vectors, respectively, ...

http://pdfs.journals.lww.com/anesthesiology/2000/10000/Effects_of_Gaseous_Anesthetics_Nitrous_Oxide_and.34.pdf

used with pBluescript vector-specific primer (r-vec, AGC. GGA TAA CAA TTT CAC ACA) to amplify and clone approximately two-thirds of the 5' end of the cDNA ...

http://www.plantphysiol.org/cgi/reprint/107/3/1019.pdf

into smaller fragments and subcloned into pBluescript vectors (Stratagene). One subclone containing a 2.1-kb HindIII fragment of the CD72 gene was ...

http://www.jimmunol.org/cgi/reprint/160/5/2287.pdf

with only the vector pBluescript ; BAT2 (control +) : strain JC201 transformed with the vector pBluescript containing the plastidial LPAAT ...

http://www.mete.mtesz.hu/pls/proceedings/eloadasok-pdf/p-1-18-maisonneuve.pdf

by BM Lange - 2000 - Cited by 154

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC16033/pdf/pq002934.pdf

by R Schreiber - 1999 - Cited by 71

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC21860/pdf/pq005310.pdf

by T Heidorn - Related articles

http://www.cder.dz/A2H2/Medias/Download/Proc PDF/PARALLEL SESSIONS/[S09] Production - Algae/15-06-06/258.pdf

in the pBluescript vectors. at Google Indexer on February 26, 2009 endo.endojournals.org .... products into pBluescript vectors to make antisense com- ...

http://endo.endojournals.org/cgi/reprint/136/3/873.pdf

by P Strass - 1993 - Related articles

http://www.weizmann.ac.il/mcb/Geiger/pages/PDFfiles/Strasser_FEBS_Let_2_93_155.pdf

by M Kano - 2003 - Cited by 8

http://medical.med.tokushima-u.ac.jp/jmi/vol50/pdf/v50_n1-2_p039.pdf

by H Nomoto - Cited by 4

http://www.lib.okayama-u.ac.jp/www/acta/pdf/56_1_25.pdf

by S Fukumoto - 2002The insert P30 gene in pBluescript SK(+) vector was subcloned into the pGEMEX-2 and pGEX4T-3 plasmids of bacterial expression vectors after digestion with ...

http://ir.obihiro.ac.jp/dspace/bitstream/10322/624/1/Prot.Vol.12-1,2-3.pdf

into the plasmid vector, pBluescript II, and sequenced. Southern Blot Analysis. Genomic DNA of P. berghei was di- gested with restriction enzymes, ...

http://jem.rupress.org/cgi/reprint/189/12/1947.pdf

fragments were also cloned into pBluescript SK vector. Sequence analysis of M. circinelloides gpd gene. Construction of several subclones and designing a ...

http://www.akademiai.com/index/PH41330770416621.pdf

pBluescript vectors with DNA directly isolated from the library screening. Lane 1, E. coli SOLR cells without pBluescript; lane 2, induced ...

http://www.ajtmh.org/cgi/reprint/62/1/99.pdf

partial oigin domains so that the excision of the plasrrid DNA from the lambda ZAP vector would generate a complete pBluescript SK(-) phagemid, including an ...

http://batzerlab.lsu.edu/hum_mol_gen_lectures/short_et_al_1988_-_lambda_zap.pdf

pBluescript vectors. pGEM vectors. pTZ vectors. pJET vectors. pBR322 and derivatives. pACYC and derivatives. pSC101 and derivatives ...

http://www.fermentas.com/templates/files/tiny_mce/coa_pdf/coa_k0503.pdf

by J Han - Cited by 122

http://gpilsinside.umaryland.edu/Courses/GPLS616 (prior years)/2007/Lecture 11/Han et al supp data.pdf